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SRX3203746: GSM2789847: Sample84H_0M; Aspergillus glaucus; RNA-Seq
1 ILLUMINA (Illumina HiSeq 2000) run: 23.3M spots, 1.1G bases, 757.5Mb downloads

Submitted by: NCBI (GEO)
Study: Comparative genomics analysis of Aspergillus glaucus CCHA provides insights into the adaptation of stress response
show Abstracthide Abstract
Aspergillus display an amazing level of diversity in physiologies, and environments that they occupy. Strategies for coping with diverse environmental stresses have evolved in different Aspergillus species. Therefore, Aspergillus are considered to be good models for investigating the adaptation and response to many natural and anthropogenic environmental stressors. Recent genome sequencing projects in several Aspergillus have provided insights into the molecular and genetic mechanisms underlying their responses to some environmental stressors. However, to better clarify the conserved and differentiated features of the adaptive response to specific stresses and to trace the evolutionary process of environmental adaptation and response in Aspergillus, insight from more Aspergillus species with different evolutionary positions, such as A. glaucus, and thus offer a large number of models of adaptation and response to various environmental stresses. Here, we report a high-quality reference genome assembly of A. glaucus CCHA from the surface of wild vegetation around saltern of Jilin, China, based on sequence data from whole-genome shotgun (WGS) sequencing platforms of Illumina solexa technologies. This assembly contains 106 scaffolds ( >1 Kb; N50 = ~0.795 Mb), has a length of ~28.9 Mb and covers ~97% of the predicted genome size (~120 Mb). Together with the data analyses from comprehensive transcriptomic surveys and comparative genomic analyses, we aim to obtain new insights into molecular mechanisms of the adaptation to living at high salt in the saltern Overall design: RNA was extracted from cultures of Aspergillus glaucus CCHA growing in medium supplemented with NaCl on different concentration.
Sample: Sample84H_0M
SAMN07682241 • SRS2530089 • All experiments • All runs
Library:
Instrument: Illumina HiSeq 2000
Strategy: RNA-Seq
Source: TRANSCRIPTOMIC
Selection: cDNA
Layout: SINGLE
Construction protocol: Mycelia were harvested by vacuum filtration with Whatman filter paper, frozen immediately in liquid nitrogen. Total RNA from frozen samples was isolated using TRIzol reagent and an additional clean-up was performed for DNA degradation using the RNeasy mini kit (Qiagen) RNA libraries were prepared for sequencing using standard Illumina protocols
Experiment attributes:
GEO Accession: GSM2789847
Links:
Runs: 1 run, 23.3M spots, 1.1G bases, 757.5Mb
Run# of Spots# of BasesSizePublished
SRR605696923,277,1921.1G757.5Mb2017-10-11

ID:
4510624

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